Papers nuevos sobre Desarrollo y células madre
78 papers nuevos sobre desarrollo y células madre en los últimos 7 días, dentro de Biología. Acá están los 50 que Pipette considera más valiosos, con el resultado principal en palabras de sus autores.
Lo mejor de la semana
Lifespan single-cell transcriptomic atlas of the human prefrontal cortex
Here, using a single-nucleus transcriptomic atlas spanning the human lifespan, we show that the dorsolateral prefrontal cortex exhibits non-linear, cell-type-specific transcriptional trajectories characterized by dynamic remodelling during development, relative stability in midlife and selective molecular reactivation in late adulthood.
Revista con revisión por paresDice ser un gran avanceLongitudinal, Non-Invasive Imaging of the Developing Chick Heart, Vasculature, and Chorioallantoic Membrane (CAM)
We present an automated laser speckle contrast imaging (LSCI) platform for non-invasive, longitudinal, label-free imaging of the developing heart and extraembryonic vasculature of the chick embryo through the intact eggshell.
PreprintAtlas of cell types and regulatory elements underlying human facial diversity
Here we created a multimodal facial atlas across embryonic weeks 6-11, providing single-cell transcriptomics, chromatin accessibility and spatial transcriptomics, all at single-cell resolution.
Revista con revisión por paresSpace partitioning by self-organized epithelial networks
Here we show that epithelial cells from multiple branching organs spontaneously self-organize into extended reticulate networks in simplified environments, revealing a conserved network-forming capacity.
PreprintAfirmaciones fuertes, leer con cuidadoPrimordial endocrine cells uncouple sexual differentiation from sex-chromosomes
Using the freshwater prawn as an exceptional case of sexual plasticity, where AG-cell transfer yields functional neo-males bearing only female sex-chromosomes, we show, through cellular-resolution approaches, that the AG arises from primordial cells present in every individual, indicating autosomal machinery of sexual differentiation completely independent from the sex-chromosomes.
PreprintAfirmaciones fuertes, leer con cuidadoDermal bone grows by cellular invasion and intercalary biomineralization
We uncover an invasive mechanism incompatible with apposition.
PreprintDice ser un gran avanceRecording proteome dynamics in the late-stage embryo at hour-timescale, spatial, and single-cell resolution with MEMBRYO
Here we present MEMBRYO: a method for continuous stable isotope labeling (SILAC) of newly synthesized proteins throughout the live embryo at the hour-timescales of developmental phenotypes.
PreprintTime-resolved single-cell state tracing exposes bidirectional state dynamics during differentiation
We show that retrospective transcriptional histories and current states are consistent with bidirectional movements along differentiation trajectories both in vivo and in vitro that become rarer as cells mature.
PreprintGPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State
Here we identify a palmitoleate-binding pocket in mammalian glypican-6 (GPC6), the gene mutated in the skeletal dysplasia omodysplasia, and show that shielding of the WNT5A lipid by GPC6 is required for gradient formation.
PreprintLsp2 links early-life diet to adult translation and lifespan in Drosophila
These findings identify Lsp2 as a molecular carrier of nutritional history across developmental transitions, linking juvenile nutritional status to adult translational capacity and lifespan.
Revista con revisión por paresLoss of TCA cycle turning promotes stem cell function
Here we use hematopoiesis, the most proliferative system in the body, as a model to show that, contrary to common assumptions, TCA cycle turning is dispensable for respiration, survival, and proliferation of stem and progenitor cells in vivo, and its loss promotes stem cell function.
PreprintA germline-specific reporter reveals conserved primordial germ cell developmental dynamics between jawless and jawed fish species
Here, we report the development of the first germline-specific reporter (GSR) in the sea lamprey (Petromyzon marinus), Tg(piwil1: egfp-UTRnanos1), a piwil1-based germline reporter that is driven by the sea lamprey piwil1 regulatory region that directs Green Fluorescent Protein (GFP) expression in early PGCs.
PreprintAfirmaciones fuertes, leer con cuidadoDice ser un gran avanceSpatiotemporal atlas resolves the mesenchymal-epithelial stem cell axis governing human hair follicle aging and pathology
Here, we integrate a multimodal spatiotemporal atlas with functional validation to define the mesenchymal-epithelial stem cell dynamics governing human hair follicle homeostasis, aging, and androgenetic alopecia.
PreprintIn utero barcoding of mouse mesoderm reveals the clonal architecture of organ mesenchyme
This approach enables Cre-independent manipulation of mammalian mesoderm and resolves organ mesenchyme development at clonal resolution.
PreprintDecoding subtype development and function in human pluripotent stem cell-derived midbrain dopaminergic neurons
Here we establish a strategy to derive substantia nigra (A9) and ventral tegmental area (A10) mDA neurons from human pluripotent stem cells (hPSCs).
PreprintFolate deficiency disrupts key metabolic transitions within the developing neural ectoderm
By analyzing folate-deficient embryogenesis from E7.0 to E9.0, we identify a transient state within the nascent neural lineage that is acutely sensitive to folate availability, leading to an interconnected disruption between key bioenergetic pathways and de novo purine biosynthesis.
PreprintLactate Receptor Activation Alleviates Senescence and Preserves Homeostasis of Aged Arteries
Together, these findings identify age-associated loss of GPR81 as a driver of vascular metabolic dysfunction and cellular senescence and establish pharmacological GPR81 activation as a promising therapeutic strategy for preserving vascular homeostasis and mitigating age-associated cardiovascular disease.
PreprintUso en el mundo realDynamic remodeling of the transcriptional landscape drives Merkel cell specification and mechanosensory differentiation
Together, our work delineates the transcriptional network orchestrating MC fate determination and mechanosensory differentiation, providing not only mechanistic insights but also highlighting actionable targets relevant to MC carcinoma and sensory restoration.
Revista con revisión por paresAn NR2F1-dependent retinoic acid network controls retinal specialization in mice and reveals foveal hypoplasia in patients with BBSOAS
These findings uncover a previously unrecognized retina-intrinsic component of BBSOAS visual pathology and establish an NR2F1-RA/CYP26A1 regulatory axis linking developmental retinal regionalization to human foveal specialization.
PreprintUso en el mundo realMultiomics Characterization Identifies S1P-secreting USSHigh Skeletal Muscle Stem Cells as Essential Drivers of Niche Remodeling and Muscle Regeneration
Altogether our findings define a senescent-like MuSC population emerging in acute injury induced muscle regeneration and provide definitive evidence to demonstrate the positive role of these cells in enhancing muscle repair via their robust secretory phenotypes and inter-cellular communicative capacity.
PreprintSerotonergic Control of Spine Morphology During Growth in Zebrafish
Here, using zebrafish, we show that serotonergic signaling is required for normal spine morphology.
PreprintGlobal proteomic analysis of sea urchin embryos reveals dynamic changes throughout embryogenesis
Overall, this study provides a comprehensive analysis of proteomic changes occurring throughout sea urchin development and establishes a powerful and sensitive high-throughput approach suitable for detecting proteomic changes with a small number of embryos.
PreprintSingle-cell TimeLapse-seq reveals MTA is an essential retrotransposon orchestrating mouse zygotic genome activation
Together, our study provides a high-resolution nascent transcriptome landscape of mouse ZGA and reveals vital regulatory roles of MTA retrotransposon in orchestrating early embryonic development.
Revista con revisión por paresAfirmaciones fuertes, leer con cuidadoTRAF7 mutations stabilize K/NRAS and hyperactivate MAPK signaling to cause CAFDADD neurodevelopmental defects
Together, our findings identify TRAF7 as a critical posttranslational regulator of RAS proteostasis during development and uncover a pivotal mechanistic link between impaired RAS ubiquitination and CAFDADD pathogenesis, providing preliminary clinical-front evidence for targeting the MAPK pathway to manage ongoing developmental deficits in TRAF7-associated disorders.
Revista con revisión por paresUso en el mundo realHeat exposure and adverse pregnancy outcomes: evidence from humans and other viviparous species
Conclusions: Overall, literature presents that heat exposure increases risk of adverse birth outcomes across studies, and taxa.
PreprintUso en el mundo realTranscriptional coupling of metabolism and cell mechanics controls humanneuroepithelium morphogenesis
These findings establish mannose metabolism as a PAX3-regulated pathway conferring mechanical competence to developing tissues.
PreprintTopological Inference for Organoids
Together, these results establish a non-destructive, image-based pipeline for estimating otherwise inaccessible physical parameters governing lumen formation and highlight the potential of topological representations for linking complex biological morphology to mechanistic models.
PreprintCombinatorial mechanisms specify cellular location and neurotransmitter identity during planarian neurogenesis
Our results demonstrate that planarian neurogenesis requires coordination of factors that initiate neurotransmitter choice and regional location.
Revista con revisión por paresKainate receptors coordinate primitive hematopoiesis and hemogenic niche organization to promote hematopoietic stem cell development
Here, we identify a developmental role for kainate receptors (KARs), glutamate-gated ion channels classically associated with excitatory neurotransmission, in coordinating primitive hematopoiesis and hemogenic niche formation in zebrafish.
PreprintPulsed-SILAC in single mouse embryos reveals early embryonic protein synthesis dynamics and phosphosite regulation
Together, single embryo pSILAC revealed a maternal proteome that is continuously synthesized, recycled, and post-translationally regulated during early embryogenesis.
PreprintRegeneration programs buffer genetic defects in animal development
Our findings provide evidence that regeneration programs are harnessed in response to developmental defects caused by genetic mutations, potentially buffering deleterious phenotypes.
Revista con revisión por paresModeling human embryonic adrenogenesis in pluripotentstem cell-derived corticomedullar-like assembloids
This work establishes a platform for modeling the reciprocal signaling relationships that drive adrenal gland development and function in health and disease and provides an approach for generating hPSC-derived adrenal cells/tissues that can be applied therapeutically.
PreprintAfirmaciones fuertes, leer con cuidadoUso en el mundo realINTACT-olotl: A simplified system for rapid cell type-specific profiling during axolotl limb regeneration
Relative to dissociation and FACS, this recovers roughly 30-fold more target nuclei in less than half the time and without a cytometer.
PreprintTranslation fidelity mechanisms and their changes in aging
Aging experiments in our two systems reveal that, with age, error frequencies increase in post-mitotic cells, but not so in mitotic ones.
PreprintDuctal myofibroblasts reactivate contractile program to stabilize alveolar architecture during lung regeneration
Together, these findings indicate ductal myofibroblasts as a developmentally programmed cell population that reactivate a contractile program to structurally support the regeneration of adult lungs.
PreprintSonic Hedgehog Is An Important Regulator Of Intervertebral Disc Homeostasis And Rejuvenation
These findings indicate that Sonic hedgehog, a developmental signal retained in the adult NP, is functionally active and required for disc homeostasis during aging and supports activation of hedgehog signaling as a candidate disease-modifying pathway for intervertebral disc pathologies.
PreprintDepletion of the isocitrate dehydrogenase 3 subunit IDHA-1 licenses germ cell-to-neuron direct reprogramming in C. elegans
Here we characterize the subunit of the mitochondrial isocitrate dehydrogenase 3 complex (IDH3), IDHA-1, as a barrier to DR of germ cells into neurons.
PreprintA blastema in sea star larvae integrates wound signaling to drive regeneration-specific and developmental gene expression patterns.
By integrating single-nucleus transcriptomics with chromatin accessibility profiling across development and regeneration, we identify a regeneration-induced blastema cell state that is molecularly distinct from pre-existing larval populations and serves as the source of regenerated tissues.
PreprintCellular basis of accelerated whole-tooth regeneration
Overall, this study provides insight into the trajectory of cellular interactions accompanying whole-tooth replacement and offers a comparative foundation for understanding dental regeneration in vertebrates.
Revista con revisión por paresAge-Related Remodeling of Cross-Tissue Transcriptional Coordination in the Human Motor System
Our analyses indicate that transcriptional aging of the human motor system is characterized by tissue-specific responses built upon a broadly shared molecular basis of aging, together with age-dependent reorganization of cross-tissue coordination and selective late-life decoupling.
PreprintHierarchical chromatin polyvalency governs robust gene regulation and organogenesis
Here, through temporal epigenomic profiling of post-implantation mouse embryos and use of the protein degradation tag (dTAG) system, we demonstrate that H2Aub, but not H3K27me3, functions as the major repressor.
PreprintThe transcription factor THAP11 is a general regulator of early embryonic polarization
Here, we demonstrate that transcriptional factor THAP11 is such a general regulator.
PreprintEcdysteroid signaling acts as a central switch underlying wing polyphenism in two hemipteran insects
Here, we find that the steroid hormone ecdysteroids serve as a central hormonal switch that orchestrates wing polyphenism through dual downstream integration of the prothoracicotropic hormone (PTTH)-its receptor (Torso) and insulin/IGF-1 signaling (IIS) pathways in both the firebug Pyrrhocoris apterus and planthopper Nilaparvata lugens , two evolutionarily divergent Hemiptera species.
Revista con revisión por paresAfirmaciones fuertes, leer con cuidadoRspo1 dependent WNT signaling safeguards ovarian development by suppressing androgen signaling
Our findings identify RSPO1/WNT/B-catenin signaling as an essential pathway that suppresses androgen signaling to ensure proper ovarian development in mouse.
PreprintSerum metabolomics reveals signatures associated with physical resilience trajectories from middle to older age
We identified metabolites across multiple chemical classes, including acylcarnitines, glutamine conjugates, and phosphocholines, that were differentially associated with physical resilience status.
PreprintMidbody formation triggers asymmetric fate specification in neural stem cells
Here we show that the midbody impedes fate determinant diffusion across the cytokinetic pore, allowing asymmetric fate specification to begin before cell division completes.
Revista con revisión por paresExtracellular matrix properties and dynamics are important for butterfly scale nanostructure development
In particular, some highly unusual scale morphologies are observed in miniature knockout individuals, suggesting a previously undescribed role of ZP domain proteins in structuring the ECM of scale cells and in mediating the mechanochemical interactions needed to produce the complex extracellular structures important for biological function in this system.
PreprintSpatio-temporal visualisation and manipulation of endogenous Yap1 in medaka
We generate and characterise a Yap1- mGreenLantern (Yap1-mGL) endogenous CRISPR/Cas9-mediated knock-in line that enables 4D analysis of Yap1 dynamics in medaka.
PreprintMATR3 is essential for oocyte growth and maturation quality through a dual molecular mechanism
These findings reveal a critical role of MATR3 in orchestrating transcription and paracrine signaling during oogenesis and suggest its potential as a diagnostic and therapeutic target for OMA.
Revista con revisión por paresUso en el mundo realSustained 10% Oxygen Promotes Atrial Rather Than Ventricular Specification During Human iPSC-Cardiomyocyte Differentiation
Results: We demonstrate by single cell RNA sequencing, and data integration with datasets for human cardiomyocytes from the different heart chambers as well as cell morphology-, ploidy-, and functional studies, that sustained 10% O2 throughout iPSC-CM differentiation promotes atrial- instead of ventricular iPSC-CM subtype specification.
PreprintUso en el mundo real