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Molecular Programming of Steric Tags for Controlled Nanopore Translocation toward Precision Clinical Diagnostic Assistance

Yiheng Liu, Shijun Lin, Minglun Li, Hao Fang, Zhen Zhang, Ruwei Wei, Yan Zhang, Jun Yan Dai, Tao Liu, Fan Xia, Xiaoding Lou

Peer-reviewed journalReal-world use

In the authors' words

This journal does not let us republish the full abstract. Here are the two sentences Pipette selected, quoted from it. Read the rest at the publisher.

Main result
Here, we report a chemically intuitive molecular programming strategy─steric blockage-enabled slow translocation (SLOW-Trans)─that overcomes this physical limitation through the rational design of sterically demanding probe molecules.
Limitation the authors admit
As a proof-of-concept extension, the modular probe design was further adapted for PSA/KLK3 activity analysis in clinical serum samples, supporting the feasibility of extending the approach to an additional protease target.

Appeared: Sunday, September 27. Journal of the American Chemical Society. Peer-reviewed journal.

DOI: 10.1021/jacs.6c15448