Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens

Kaichun Yang; Wyatt A. Peele; Pengzhan Liu; Jianping Xia; Venkata P. Dandey; Ruoyu Zhong; Ke Jin; Kedar Sharma; Abigail J. Watson; Shujie Yang; Ying Chen; Aryan Agahtehrani; Shu Nakajima Lan; Peiran Zhang; Zhenhua Tian; Mingyuan Liu; Elizabeth G. Viverette; Xianchen Xu; Qian Wu; Mario Juan Borgnia; Luke P. Lee; Tony Jun Huang

doi:10.1126/sciadv.aeh5900

Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens

Kaichun Yang, Wyatt A. Peele, Pengzhan Liu, Jianping Xia, Venkata P. Dandey, Ruoyu Zhong, Ke Jin, Kedar Sharma, Abigail J. Watson, Shujie Yang, Ying Chen, Aryan Agahtehrani, Shu Nakajima Lan, Peiran Zhang, Zhenhua Tian, Mingyuan Liu, Elizabeth G. Viverette, Xianchen Xu, Qian Wu, Mario Juan Borgnia, Luke P. Lee, Tony Jun Huang

Peer-reviewed journalBold claims, read critically

In the authors' words

This journal does not let us republish the full abstract. Here are the two sentences Pipette selected, quoted from it. Read the rest at the publisher.

Main result

Guided by this physical mechanism, validation using liposomes and apoferritin demonstrates that ACE increases particle density by up to fourfold while preserving structural integrity and improving imaging quality.

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Appeared: Friday, September 25. Science Advances. Peer-reviewed journal.

DOI: 10.1126/sciadv.aeh5900

Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens

In the authors' words

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